Hybrid Antibody-Aptamer Assay for Detection of Tetrodotoxin in Pufferfish.

ABSTRACT: The marine toxin tetrodotoxin (TTX) poses a great risk to public health safety due to its severe paralytic effects after ingestion. Seafood poisoning caused by the consumption of contaminated marine species like pufferfish due to its expansion to nonendemic areas has increased the need for fast and reliable detection of the toxin to effectively implement prevention strategies. Liquid chromatography-mass spectrometry is considered the most accurate method, although competitive immunoassays have also been reported. In this work, we sought to develop an aptamer-based assay for the rapid, sensitive, and cost-effective detection of TTX in pufferfish. Using capture-SELEX combined with next-generation sequencing, aptamers were identified, and their binding properties were evaluated. Finally, a hi

SUBMITTER: Shkembi X 

PROVIDER: S-EPMC8581965 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature