Fabrication of MERS-nanovesicle biosensor composed of multi-functional DNA aptamer/graphene-MoS₂ nanocomposite based on electrochemical and surface-enhanced Raman spectroscopy.

ABSTRACT: Middle East respiratory syndrome coronavirus (MERS-CoV) is one of the most harmful viruses for humans in nowadays. To prevent the spread of MERS-CoV, a valid detection method is highly needed. For the first time, a MERS-nanovesicle (NV) biosensor composed of multi-functional DNA aptamer and graphene oxide encapsulated molybdenum disulfide (GO-MoS₂) hybrid nanocomposite was fabricated based on electrochemical (EC) and surface-enhanced Raman spectroscopy (SERS) techniques. The MERS-NV aptamer was designed for specifically binding to the spike protein on MERS-NVs and it is prepared using the systematic evolution of ligands by exponential enrichment (SELEX) technique. For constructing a multi-functional MERS aptamer (MF-aptamer), the prepared aptamer was connected to the DNA 3-way j

SUBMITTER: Kim G 

PROVIDER: S-EPMC8582077 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature