Functional screen of inflammatory bowel disease genes reveals key epithelial functions.

ABSTRACT:

Background

Genetic studies have been tremendously successful in identifying genomic regions associated with a wide variety of phenotypes, although the success of these studies in identifying causal genes, their variants, and their functional impacts has been more limited.

Methods

We identified 145 genes from IBD-associated genomic loci having endogenous expression within the intestinal epithelial cell compartment. We evaluated the impact of lentiviral transfer of the open reading frame (ORF) of these IBD genes into the HT-29 intestinal epithelial cell line via transcriptomic analyses. By comparing the genes in which expression was modulated by each ORF, as well as the functions enriched within these gene lists, we identified ORFs with shared impacts and their putative diseas

SUBMITTER: Ntunzwenimana JC 

PROVIDER: S-EPMC8582123 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature