Development of Surgical and Visualization Procedures to Analyze Vasculatures by Mouse Tail Edema Model.

ABSTRACT:

Background

Because of the high frequency of chronic edema formation in the current "aged" society, analyses and detailed observation of post-surgical edema are getting more required. Post-surgical examination of the dynamic vasculature including L.V. (Lymphatic Vasculature) to monitor edema formation has not been efficiently performed. Hence, procedures for investigating such vasculature are essential. By inserting transparent sheet into the cutaneous layer of mouse tails as a novel surgery model (the Tail Edema by Silicone sheet mediated Transparency protocol; TEST), the novel procedures are introduced and analyzed by series of histological analyses including video-based L.V. observation and 3D histological reconstruction of vasculatures in mouse tails.

Results

The dynamic

SUBMITTER: Kumegawa S 

PROVIDER: S-EPMC8582144 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature