Electrochemiluminescence Immunosensor Based on Nanobody and Au/CaCO₃ Synthesized Using Waste Eggshells for Ultrasensitive Detection of Ochratoxin A.

ABSTRACT: A novel ultrasensitive electrochemiluminescence (ECL) immunoassay based on Au/CaCO₃ was proposed for detecting ochratoxin A (OTA) in coffee. Au/CaCO₃ nanocomposites synthesized using waste eggshells as the template with a large surface area and excellent electrochemical properties were applied for immobilizing a large amount of Ru(bpy)₃ ²⁺ and conjugating a high-affinity nanobody (prepared by the phage display technique). Coupling of the Au/CaCO₃ nanocomposites and nanobody technologies provided an ultrasensitive and highly selective ECL immunosensor for OTA detection in the range of 10 pg/mL-100 ng/mL with a low detection limit of 5.7 pg/mL. Moreover, the as-prepared ECL immunosensor showed excellent performance and high stability. Fi

SUBMITTER: Li L 

PROVIDER: S-EPMC8582264 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature