Universal functions of the σ finger in alternative σ factors during transcription initiation by bacterial RNA polymerase.

ABSTRACT: The bacterial σ factor plays the central role in promoter recognition by RNA polymerase (RNAP). The primary σ factor, involved in transcription of housekeeping genes, was also shown to participate in the initiation of RNA synthesis and promoter escape by RNAP. In the open promoter complex, the σ finger formed by σ region 3.2 directly interacts with the template DNA strand upstream of the transcription start site. Here, we analysed the role of the σ finger in transcription initiation by four alternative σ factors in Escherichia coli, σ³⁸, σ³², σ²⁸ and σ²⁴. We found that deletions of the σ finger to various extent compromise the activity of RNAP holoenzymes containing alternative σ factors, especially at low NTP concentrations. All four σs a

SUBMITTER: Oguienko A 

PROVIDER: S-EPMC8583087 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature