Post-transcriptional regulation of redox homeostasis by the small RNA SHOxi in haloarchaea.

ABSTRACT: While haloarchaea are highly resistant to oxidative stress, a comprehensive understanding of the processes regulating this remarkable response is lacking. Oxidative stress-responsive small non-coding RNAs (sRNAs) have been reported in the model archaeon, Haloferax volc anii, but targets and mechanisms have not been elucidated. Using a combination of high throughput and reverse molecular genetic approaches, we elucidated the functional role of the most up-regulated intergenic sRNA during oxidative stress in H. volcanii, named Small RNA in Haloferax Oxidative Stress (SHOxi). SHOxi was predicted to form a stable secondary structure with a conserved stem-loop region as the potential binding site for trans-targets. NAD-dependent mal

SUBMITTER: Gelsinger DR 

PROVIDER: S-EPMC8583180 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature