Real-time monitoring of Pseudomonas aeruginosa biofilm growth dynamics and persister cells' eradication.

ABSTRACT: Biofilm formation and the appearance of persister cells with low metabolic rates are key factors affecting conventional treatment failure and antibiotic resistance. Using impedance-based measurements, crystal violet staining and traditional culture we have studied the biofilm growth dynamics of 13 Pseudomonas aeruginosa strains under the effect of seven conventional antibiotics. Real-time growth quantifications revealed that the exposure of established P. aeruginosa biofilms to certain concentrations of ciprofloxacin, ceftazidime and tobramycin induced the emergence of persister cells, that showed different morphology and pigmentation, as well increased antibiotic resistance. Whole-genome sequencing of wildtype and persister cells identified several SNPs, a genomic inversion

SUBMITTER: Ziemyte M 

PROVIDER: S-EPMC8583918 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature