Ontology highlight
ABSTRACT:
SUBMITTER: Meyer JG
PROVIDER: S-EPMC8583964 | biostudies-literature | 2021
REPOSITORIES: biostudies-literature
Methods in molecular biology (Clifton, N.J.) 20210101
Shotgun proteomics is the inferential analysis of proteoforms using peptide proxies produced by enzyme-catalyzed hydrolysis of entire proteomes. Such peptides are usually identified by nanoflow liquid chromatography coupled to tandem mass spectrometry analysis (nLC-MS/MS). Traditionally, MS/MS analysis is performed in data-dependent acquisition (DDA) mode, which usually produces a pattern of fragment masses unique to a single peptide's fragmentation. Here, I describe a statistically rigorous qua ...[more]