Proteomic Landscape of Adeno-Associated Virus (AAV)-Producing HEK293 Cells.

ABSTRACT: Adeno-associated viral (AAV) vectors are widely used for gene therapy, providing treatment for diseases caused by absent or defective genes. Despite the success of gene therapy, AAV manufacturing is still challenging, with production yields being limited. With increased patient demand, improvements in host cell productivity through various engineering strategies will be necessary. Here, we study the host cell proteome of AAV5-producing HEK293 cells using reversed phase nano-liquid chromatography and tandem mass spectrometry (RPLC-MS/MS). Relative label-free quantitation (LFQ) was performed, allowing a comparison of transfected vs. untransfected cells. Gene ontology enrichment and pathway analysis revealed differential expression of proteins involved in fundamental cellular processes such a

SUBMITTER: Strasser L 

PROVIDER: S-EPMC8584267 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature