Hierarchically Structured Polystyrene-Based Surfaces Amplifying Fluorescence Signals: Cytocompatibility with Human Induced Pluripotent Stem Cell.

ABSTRACT: An innovative multi-step phase separation process was used to prepare tissue culture for the polystyrene-based, hierarchically structured substrates, which mimicked in vivo microenvironment and architecture. Macro- (pore area from 3000 to 18,000 µm²; roughness (Ra) 7.2 ± 0.1 µm) and meso- (pore area from 50 to 300 µm²; Ra 1.1 ± 0.1 µm) structured substrates covered with micro-pores (area around 3 µm²) were prepared and characterised. Both types of substrate were suitable for human-induced pluripotent stem cell (hiPSC) cultivation and were found to be beneficial for the induction of cardiomyogenesis in hiPSC. This was confirmed both by the number of promoted proliferated cells and the expressions of specific markers (Nkx2.5, MYH6, MYL2, and MYL7). Moreover,

SUBMITTER: Skopalova K 

PROVIDER: S-EPMC8584612 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature