Optimized workflow for human PBMC multiomic immunosurveillance studies.
Ontology highlight
ABSTRACT:
SUBMITTER: Genge PC
PROVIDER: S-EPMC8585660 | biostudies-literature | 2021 Dec
REPOSITORIES: biostudies-literature
Publications
Optimized workflow for human PBMC multiomic immunosurveillance studies.
Genge Palak C PC Roll Charles R CR Heubeck Alexander T AT Swanson Elliott E Kondza Nina N Lord Cara C Weiss Morgan M Hernandez Veronica V Phalen Cole C Thomson Zachary Z Torgerson Troy R TR Skene Peter J PJ Bumol Thomas F TF Reading Julian J
STAR protocols 20211105 4
Deep immune profiling is essential for understanding the human immune system in health and disease. Successful biological interpretation of this data requires consistent laboratory processing with minimal batch-to-batch variation. Here, we detail a robust pipeline for the profiling of human peripheral blood mononuclear cells by both high-dimensional flow cytometry and single-cell RNA-seq. These protocols reduce batch effects, generate reproducible data, and increase throughput. For complete deta ...[more]
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