Stability of proteins encapsulated in Michael-type addition polyethylene glycol hydrogels.

ABSTRACT: Degradable polyethylene glycol (PEG) hydrogels are excellent vehicles for sustained drug release due to their biocompatibility, tunable physical properties, and customizable degradation. However, protein therapeutics are unstable under physiological conditions and releasing degraded or inactive therapeutics can induce immunogenic effects. While controlling protein release from PEG hydrogels has been extensively investigated, few studies have detailed protein stability long-term or under stress conditions. Here, lysozyme and alcohol dehydrogenase (ADH) stability were explored upon encapsulation in PEG hydrogels formed through Michael-type addition. The stability and structure of the two model proteins were monitored by measuring the free energy of unfolding and fluoresce quenching when conf

SUBMITTER: Ghassemi Z 

PROVIDER: S-EPMC8585711 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature