Endothelial cell-specific reduction of heparan sulfate suppresses glioma growth in mice.

ABSTRACT:

Purpose

Heparan sulfate (HS) is one of the factors that has been suggested to be associated with angiogenesis and invasion of glioblastoma (GBM), an aggressive and fast-growing brain tumor. However, it remains unclear how HS of endothelial cells is involved in angiogenesis in glioblastoma and its prognosis. Thus, we investigated the effect of endothelial cell HS on GBM development.

Methods

We generated endothelial cell-specific knockout of Ext1, a gene encoding a glycosyltransferase and essential for HS synthesis, and murine GL261 glioblastoma cells were orthotopically transplanted. Two weeks after transplantation, we examined the tumor progression and underlying mechanisms.

Results

The endothelial cell-specific Ext1 knockout (Ext1 ^CKO

SUBMITTER: Kinoshita T 

PROVIDER: S-EPMC8585801 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature