Quantitative analysis of asynchronous transcription-translation and transcription processivity in Bacillus subtilis under various growth conditions.

ABSTRACT: Tight coordination between transcription and translation has long been recognized as the hallmark of gene expression in bacteria. In Escherichia coli cells, disruption of the transcription-translation coordination leads to the loss of transcription processivity via triggering Rho-mediated premature transcription termination. Here we quantitatively characterize the transcription and translation kinetics in Gram-positive model bacterium Bacillus subtilis. We found that the speed of transcription elongation is much faster than that of translation elongation in B. subtilis under various growth conditions. Moreover, a Rho-independent loss of transcription processivity occurs constitutively in several genes/operons but is not subject to translational control. When the transc

SUBMITTER: Zhu M 

PROVIDER: S-EPMC8586808 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature