Solid-State NMR Study to Probe the Effects of Divalent Metal Ions (Ca²⁺ and Mg²⁺) on the Magnetic Alignment of Polymer-Based Lipid Nanodiscs.

ABSTRACT: Divalent cations, especially Ca²⁺ and Mg²⁺, play a vital role in the function of biomolecules and making them important to be constituents in samples for in vitro biophysical and biochemical characterizations. Although lipid nanodiscs are becoming valuable tools for structural biology studies on membrane proteins and for drug delivery, most types of nanodiscs used in these studies are unstable in the presence of divalent metal ions. To avoid the interaction of divalent metal ions with the belt of the nanodiscs, synthetic polymers have been designed and demonstrated to form stable lipid nanodiscs under such unstable conditions. Such polymer-based nanodiscs have been shown to provide an ideal platform for structural studies using both solid-state and solution NMR spectr

SUBMITTER: Ravula T 

PROVIDER: S-EPMC8587631 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature