Unknown

Dataset Information

0

Evaluation of the Inhibitory Effects of Pyridylpyrazole Derivatives on LPS-Induced PGE2 Productions and Nitric Oxide in Murine RAW 264.7 Macrophages.

ABSTRACT: A series of thirteen triarylpyrazole analogs were investigated as inhibitors of lipopolysaccharide (LPS)-induced prostaglandin E2 (PGE2) and nitric oxide (NO) production in RAW 264.7 macrophages. The target compounds 1a-m have first been assessed for cytotoxicity against RAW 264.7 macrophages to determine their non-cytotoxic concentration(s) for anti-inflammatory testing to make sure that the inhibition of PGE2 and NO production would not be caused by cytotoxicity. It was found that compounds 1f and 1m were the most potent PGE2 inhibitors with IC50 values of 7.1 and 1.1 μM, respectively. In addition, these compounds also showed inhibitory effects of 11.6% and 37.19% on LPS-induced NO production, respectively. The western blots analysis of COX-2 and iNOS showed that the PGE2 and NO inhibitory effect of compound 1m are attributed to inhibition of COX-2 and iNOS protein expression through inactivation of p38.

SUBMITTER: Gamal El-Din MM 

PROVIDER: S-EPMC8587869 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Evaluation of the Inhibitory Effects of Pyridylpyrazole Derivatives on LPS-Induced PGE<sub>2</sub> Productions and Nitric Oxide in Murine RAW 264.7 Macrophages.

Gamal El-Din Mahmoud M MM   El-Gamal Mohammed I MI   Kwon Young-Do YD   Kim Su-Yeon SY   Han Hee-Soo HS   Park Sang-Eun SE   Oh Chang-Hyun CH   Lee Kyung-Tae KT   Kim Hee-Kwon HK  

Molecules (Basel, Switzerland) 20211027 21

A series of thirteen triarylpyrazole analogs were investigated as inhibitors of lipopolysaccharide (LPS)-induced prostaglandin E<sub>2</sub> (PGE<sub>2</sub>) and nitric oxide (NO) production in RAW 264.7 macrophages. The target compounds <b>1a</b>-<b>m</b> have first been assessed for cytotoxicity against RAW 264.7 macrophages to determine their non-cytotoxic concentration(s) for anti-inflammatory testing to make sure that the inhibition of PGE<sub>2</sub> and NO production would not be caused  ...[more]

Similar Datasets

Unknown Synthesis of New Triarylpyrazole Derivatives Possessing Terminal Sulfonamide Moiety and Their Inhibitory Effects on PGE₂ and Nitric Oxide Productions in Lipopolysaccharide-Induced RAW 264.7 Macrophages.
| S-EPMC6222820 | biostudies-literature
Unknown Carnosine modulates nitric oxide in stimulated murine RAW 264.7 macrophages.
| S-EPMC5697141 | biostudies-literature
Unknown Synthesis of Halogenated 1,5-Diarylimidazoles and Their Inhibitory Effects on LPS-Induced PGE2 Production in RAW 264.7 Cells.
| S-EPMC8538130 | biostudies-literature
Unknown Aminothiazoles inhibit RANKL- and LPS-mediated osteoclastogenesis and PGE2 production in RAW 264.7 cells.
| S-EPMC4882984 | biostudies-literature
Transcriptomics Gene expression of RAW 264.7 cells treated with LPS, ISO, PGE2, LPS PLUS ISO, and LPS PLUS PGE2
2006-06-06 | GSE4964 | GEO
Transcriptomics LPS stimulation of Mouse (RAW 264.7) macrophages
2016-02-25 | GSE76562 | GEO
Transcriptomics LPS stimulation of Mouse (RAW 264.7) macrophages
2016-02-25 | E-GEOD-76562 | biostudies-arrayexpress
Unknown Thiazolidinedione Derivative Suppresses LPS-induced COX-2 Expression and NO Production in RAW 264.7 Macrophages.
| S-EPMC6934980 | biostudies-literature
Unknown Anti-Inflammatory Activity and ROS Regulation Effect of Sinapaldehyde in LPS-Stimulated RAW 264.7 Macrophages.
| S-EPMC7570554 | biostudies-literature
Unknown Vernonia polysphaera Baker: Anti-inflammatory activity in vivo and inhibitory effect in LPS-stimulated RAW 264.7 cells.
| S-EPMC6907817 | biostudies-literature