Proteomic and Bioinformatic Analysis of Decellularized Pancreatic Extracellular Matrices.

ABSTRACT: Tissue microenvironments are rich in signaling molecules. However, factors in the tissue matrix that can serve as tissue-specific cues for engineering pancreatic tissues have not been thoroughly identified. In this study, we performed a comprehensive proteomic analysis of porcine decellularized pancreatic extracellular matrix (dpECM). By profiling dpECM collected from subjects of different ages and genders, we showed that the detergent-free decellularization method developed in this study permits the preservation of approximately 62.4% more proteins than a detergent-based method. In addition, we demonstrated that dpECM prepared from young pigs contained approximately 68.5% more extracellular matrix proteins than those prepared from adult pigs. Furthermore, we categorized dpECM proteins by

SUBMITTER: Hu M 

PROVIDER: S-EPMC8588251 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature