Engineered Fully Human Single-Chain Monoclonal Antibodies to PIM2 Kinase.

ABSTRACT: Proviral integration site of Moloney virus-2 (PIM2) is overexpressed in multiple human cancer cells and high level is related to poor prognosis; thus, PIM2 kinase is a rational target of anti-cancer therapeutics. Several chemical inhibitors targeting PIMs/PIM2 or their downstream signaling molecules have been developed for treatment of different cancers. However, their off-target toxicity is common in clinical trials, so they could not be advanced to official approval for clinical application. Here, we produced human single-chain antibody fragments (HuscFvs) to PIM2 by using phage display library, which was constructed in a way that a portion of phages in the library carried HuscFvs against human own proteins on their surface with the respective antibody genes in the phage genome. Bacteria

SUBMITTER: Kaewchim K 

PROVIDER: S-EPMC8588357 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature