Selection and Characterization of Vimentin-Binding Aptamer Motifs for Ovarian Cancer.

ABSTRACT: The application of aptamers in biomedicine is emerging as an essential technology in the field of cancer research. As small single-stranded DNA or RNA ligands with high specificity and low immunogenicity for their targets, aptamers provide many advantages in cancer therapeutics over protein-based molecules, such as antibodies. Vimentin is an intermediate filament protein that is overexpressed in endothelial cells of cancerous tissue. High expression levels of vimentin have been associated with increased capacity for migration and invasion of the tumor cells. We have selected and identified thioated aptamers with high specificity for vimentin using human ovarian cancer tissues. Tentative binding motifs were chosen for two vimentin aptamers based on predicted secondary structures. Each of th

SUBMITTER: Costello AM 

PROVIDER: S-EPMC8588432 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature