Zika virus and temperature modulate Elizabethkingia anophelis in Aedes albopictus.

ABSTRACT:

Background

Vector-borne pathogens must survive and replicate in the hostile environment of an insect's midgut before successful dissemination. Midgut microbiota interfere with pathogen infection by activating the basal immunity of the mosquito and by synthesizing pathogen-inhibitory metabolites.

Methods

The goal of this study was to assess the influence of Zika virus (ZIKV) infection and increased temperature on Aedes albopictus midgut microbiota. Aedes albopictus were reared at diurnal temperatures of day 28 °C/night 24 °C (L) or day 30 °C/night 26 °C (M). The mosquitoes were given infectious blood meals with 2.0 × 10⁸ PFU/ml ZIKV, and 16S rRNA sequencing was performed on midguts at 7 days post-infectious blood meal exposure.

Results

Our findings demonstra

SUBMITTER: Onyango MG 

PROVIDER: S-EPMC8588690 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature