MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression.
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ABSTRACT: Background
So far, few have concerned miR-497-5p in lung squamous cell carcinoma (LUSC).Methods
MiR-497-5p expression in LUSC was measured by qRT-PCR. Its impacts on tumor-related cell behaviors were investigated by CCK8 assay, scratch healing assay, flow cytometry and Transwell invasion methods. In addition, interaction between miR-497-5p and CDCA4 in LUSC was also elucidated through rescue experiment, western blot, dual-luciferase, and bioinformatics analysis.Results
Low level of miR-497-5p was confirmed in LUSC tissue and cells. Overexpressed miR-497-5p markedly inhibited cancer progression. miR-497-5p restrained CDCA4 expression. Rescue assay showed that overexpressing miR-497-5p eliminated effect of overexpressed CDCA4.Conclusion
By targeting CDCA4, miR-497-5p restrained development of LUSC.
SUBMITTER: Hu J
PROVIDER: S-EPMC8588708 | biostudies-literature | 2021 Nov
REPOSITORIES: biostudies-literature
Publications
MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression.
Hu Jiangwei J Xiang Xinqin X Guan Wei W Lou Weihua W He Junming J Chen Jian J Fu Yin Y Lou Guoliang G
Journal of cardiothoracic surgery 20211112 1
<h4>Background</h4>So far, few have concerned miR-497-5p in lung squamous cell carcinoma (LUSC).<h4>Methods</h4>MiR-497-5p expression in LUSC was measured by qRT-PCR. Its impacts on tumor-related cell behaviors were investigated by CCK8 assay, scratch healing assay, flow cytometry and Transwell invasion methods. In addition, interaction between miR-497-5p and CDCA4 in LUSC was also elucidated through rescue experiment, western blot, dual-luciferase, and bioinformatics analysis.<h4>Results</h4>Lo ...[more]
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