Palmdelphin Regulates Nuclear Resilience to Mechanical Stress in the Endothelium.

ABSTRACT:

Background

PALMD (palmdelphin) belongs to the family of paralemmin proteins implicated in cytoskeletal regulation. Single nucleotide polymorphisms in the PALMD locus that result in reduced expression are strong risk factors for development of calcific aortic valve stenosis and predict severity of the disease.

Methods

Immunodetection and public database screening showed dominant expression of PALMD in endothelial cells (ECs) in brain and cardiovascular tissues including aortic valves. Mass spectrometry, coimmunoprecipitation, and immunofluorescent staining allowed identification of PALMD partners. The consequence of loss of PALMD expression was assessed in small interferring RNA-treated EC cultures, knockout mice, and human valve samples. RNA sequencing of ECs and tran

SUBMITTER: Sainz-Jaspeado M 

PROVIDER: S-EPMC8589083 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature