Evaluation of protein kinase D auto-phosphorylation as biomarker for NLRP3 inflammasome activation.

ABSTRACT:

Background

The NLRP3 inflammasome is a critical component of sterile inflammation, which is involved in many diseases. However, there is currently no known proximal biomarker for measuring NLRP3 activation in pathological conditions. Protein kinase D (PKD) has emerged as an important NLRP3 kinase that catalyzes the release of a phosphorylated NLRP3 species that is competent for inflammasome complex assembly.

Methods

To explore the potential for PKD activation to serve as a selective biomarker of the NLRP3 pathway, we tested various stimulatory conditions in THP-1 and U937 cell lines, probing the inflammasome space beyond NLRP3. We analyzed the correlation between PKD activation (monitored by its auto-phosphorylation) and functional inflammasome readouts.

Results

PKD a

SUBMITTER: Heiser D 

PROVIDER: S-EPMC8589197 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature