Soluble CD127 potentiates IL-7 activity in vivo in healthy mice.

ABSTRACT:

Introduction

Soluble forms of cytokine receptors can be involved in the endogenous regulation of cytokine activity. Soluble interleukin 7 receptor α (sCD127) naturally binds IL-7, therefore there is interest in its potential application as an immunotherapeutic agent to regulate IL-7. With the hypothesis that sCD127 enhances IL-7 activity, thus promoting T-cell proliferation in vivo, we sought to assess the effect of sCD127, IL-7 or IL-7 + sCD127 treatment on CD4⁺ and CD8⁺ T-cells in the blood and spleen of mice.

Methods

Peripheral blood mononuclear cells and splenocytes were prepared, and analyzed for T-cell number, phenotype and proliferation (Ki67⁺ ) by flow cytometry.

Results

IL-7 treatment induced T-cell proliferation, increased T-

SUBMITTER: Aloufi NA 

PROVIDER: S-EPMC8589376 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature