CircZNF124 regulates cell proliferation, leucine uptake, migration and invasion by miR-199b-5p/SLC7A5 pathway in endometrial cancer.

ABSTRACT:

Background

Recent studies have revealed that circular RNA participates in endometrial carcinoma (EC) progression. Here we investigated the role of circRNA zinc finger protein 124 (circZNF124) in EC genesis and underlying mechanism.

Methods

The expression levels of circZNF124, microRNA-199b-5p (miR-199b-5p) and solute carrier family 7 member 5 (SLC7A5) were detected by quantitative real-time polymerase chain reaction. The expression of SLC7A5 and other indicated marker proteins was determined by western blot analysis. For functional assay, cell proliferation, leucine uptake and metastasis were investigated by total cell number, cell counting kit-8, cell colony formation, leucine uptake or transwell assay. The interaction between miR-199b-5p and circZNF124 or SLC7A5 was predic

SUBMITTER: Shu L 

PROVIDER: S-EPMC8589382 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature