Neutrophil extracellular traps induce a hypercoagulable state in glioma.

ABSTRACT:

Background

Venous thromboembolism (VTE) is one of the leading complications in glioma patients. Neutrophil extracellular traps (NETs) have been reported to play a critical role in the physiopathology of cancer. We aimed to investigate the presence and potential role of NETs in the hypercoagulable state in glioma patients. Moreover, we evaluated the interaction between NETs and endothelial cells (ECs) in glioma patients.

Methods

The plasma levels of NETs were detected by enzyme-linked immunosorbent assay. The NET procoagulant activity was performed based on fibrin formation assays. The NET generation and NET-treated ECs in vitro were observed by confocal microscopy. Activated platelets (PLTs) and PLT-neutrophil aggregates were detected by flow cytometry.

Results

Plasma

SUBMITTER: Zhang S 

PROVIDER: S-EPMC8589396 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature