Interleukin-6 receptor alpha and CD27 discriminate intratumoral T helper 17 subpopulations with distinct functional properties in a mouse lung cancer model.

ABSTRACT:

Background

T helper 17 (Th17) cells actively participate in the tumor immune response in lung cancer. However, the heterogeneity and plasticity of intratumoral Th17 cells in lung cancer remain elusive. In this study, Th17 subpopulations were characterized in a mouse lung cancer model.

Methods

Urethane was administered to induce lung cancer in interleukin (IL)-17-EGFP transgenic mice. Flow cytometry was used to analyze the phenotypes, signaling status, and functions of Th17 subpopulations either in vivo or in vitro. The adoptive transfer assay and real-time polymerase chain reaction were applied to analyze the plasticity of Th17 subpopulations.

Results

IL-6Rα^high CD27^- Th17 and IL-6Rα^low CD27⁺ Th17 were identified in intratum

SUBMITTER: Liu C 

PROVIDER: S-EPMC8589402 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature