The potential role of long noncoding RNAs in primary open-angle glaucoma.

ABSTRACT:

Purpose

To identify the potential genes in human trabecular meshwork (TM) related to primary open-angle glaucoma (POAG).

Methods

First, long noncoding RNA (LncRNA) and mRNA expression profiles in TM samples from 4 control subjects and 4 POAG patients were accessed by microarray analyses. Then, twenty lncRNAs were validated by real-time quantitative PCR in the same samples from microarray analyses. Finally, eight highly expressed lncRNAs were further tested by real-time quantitative PCR in TM from 8 normal controls and 19 POAG patients. Expression data were normalized and analyzed using the R software. Pathway analyses were performed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis.

Results

A total of 2179 lncRNAs and 923 mRNAs in the T

SUBMITTER: Zhang F 

PROVIDER: S-EPMC8589759 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature