Stimulation of motilin secretion by bile, free fatty acids, and acidification in human duodenal organoids.

ABSTRACT:

Objective

Motilin is a proximal small intestinal hormone with roles in gastrointestinal motility, gallbladder emptying, and hunger initiation. In vivo motilin release is stimulated by fats, bile, and duodenal acidification but the underlying molecular mechanisms of motilin secretion remain poorly understood. This study aimed to establish the key signaling pathways involved in the regulation of secretion from human motilin-expressing M-cells.

Methods

Human duodenal organoids were CRISPR-Cas9 modified to express the fluorescent protein Venus or the Ca²⁺ sensor GCaMP7s under control of the endogenous motilin promoter. This enabled the identification and purification of M-cells for bulk RNA sequencing, peptidomics, calcium imaging, and electrophysiology. Motilin secre

SUBMITTER: Miedzybrodzka EL 

PROVIDER: S-EPMC8590067 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature