Gene profiling of Toll-like receptor signalling pathways in neutrophils of patients with acute-on-chronic liver failure.

ABSTRACT:

Objectives

Toll-like receptors (TLRs) on neutrophils play a crucial role in detecting pathogens and organ/tissue injury in acute-on-chronic liver failure (ACLF). However, little is known about the exact mechanisms and potential signalling pathways. The aim of this study was to investigate alterations in TLR signalling pathways in neutrophils of ACLF patients.

Methods

Twenty-seven patients with compensated cirrhosis (n = 9), decompensated cirrhosis (n = 9) and ACLF (n = 9) were enrolled in the study. Neutrophils were isolated, and alterations in TLR signalling pathways were evaluated using an RT² Profiler™ PCR Array. The fold change for each gene (2(^-∆∆CT)) was compared among the groups. Genes with a fold change ratio of ≥ 2 or ≤ 0.5 along with a p value

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC8590218 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature