Dnmt3a is downregulated by Stat5a and mediates G0/G1 arrest by suppressing the miR-17-5p/Cdkn1a axis in Jak2^V617F cells.

ABSTRACT:

Background

Despite of the frequently reported Dnmt3a abormality in classical myeloproliferative neoplasms (cMPNs) patients, few research explores how the Dnmt3a is regulated by Jak2^V617F mutation. In this study, we have investigated how the Dnmt3a is regulated by Jak2^V617F mutation and its effects on downstream signaling pathways in cMPNs.

Methods

Specimens of Jak2^V617F positive cMPN patients and normal controls were collected. Murine BaF3 cell line was used to construct cell models. Dual-Glo luciferase assays and chromatin immunoprecipitation (ChIP)-qPCR were performed to detect the impact of Stat5a on transcription activity of Dnmt3a. Soft agar colony formation assay and cell counting assay were performed to detect cell proliferation. BrdU

SUBMITTER: Zhou J 

PROVIDER: S-EPMC8590245 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature