ADSCs enhance VEGFR3-mediated lymphangiogenesis via METTL3-mediated VEGF-C m⁶A modification to improve wound healing of diabetic foot ulcers.

ABSTRACT:

Background

Adipose-derived mesenchymal stem cells (ADSCs) are an important focus in regenerative medicine. However, the biological function of ADSCs in the wound repair of diabetic foot ulcers (DFUs) remains unclear. This study aimed to determine the underlying mechanisms of ADSCs involved in the wound healing of DFUs.

Methods

The cell surface markers cluster of differentiation 34 (CD34), stromal cell antigen 1 (Stro-1), cluster of differentiation 90 (CD90) and cluster of differentiation 105 (CD105) on ADSCs were identified by flow cytometry. Oil Red O staining and Alizarin Red S staining were performed to identify the multipotential differentiation of ADSCs into adipocytes and bone. The levels of Methyltransferase-like 3 (METTL3), vascular endothelial growth factor C (VEGF-

SUBMITTER: Zhou J 

PROVIDER: S-EPMC8590326 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature