Purification and Characterization of an Extracellular Alkaline Solvent-stable Metalloprotease Secreted from Newly Isolated Bacillus sp. DEM05: Optimization of Protease Production

ABSTRACT:

Background:

Proteases play an important role in food, leather, detergent, and medical technologies.

Objectives:

In the current study, an alkaliphilic solvent-stable thermotolerant metalloprotease was isolated from Bacillus sp. DEM05.

Material and Methods:

For culture optimization, carbon, and nitrogen sources as well as incubation temperature, pH, and time were examined.

Results:

Herein the highest outcome for bacterial growth and protease production was obtained after 72 h incubation (pH 7) at 37 °C. DEM05 protease

SUBMITTER: Mohamadi S 

PROVIDER: S-EPMC8590717 | biostudies-literature |

REPOSITORIES: biostudies-literature