Production of Bioplastic (Polyhydroxybutyrate) with Local Bacillus megaterium Isolated from Petrochemical Wastewater.

ABSTRACT:

Background

Polyhydroxybutyrate is a biodegradable plastic produced by some bacteria and can completely be replaced with petroleum based non-degradable plastics.

Objectives

This study was done to isolate and identify one local strain with a high-production ability for industrial purposes.

Material and methods

The sampling from petrochemical wastewater was done. The existence of polyhydroxybutyrate in isolates was studied with Sudan Black staining. Using the Sudan Black B plate assay method and estimating produced PHB amount, the most potent isolate was chosen. This isolate was distinguished by morphological and biochemical methods and determining 16S rRNA gene sequencing. The final confirmation of polyhydroxybutyrate synthesis was done by FTIR and ¹H NMR. To

SUBMITTER: Amiri Kojuri S 

PROVIDER: S-EPMC8590724 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature