ROR2 has a protective role in melanoma by inhibiting Akt activity, cell-cycle progression, and proliferation.

ABSTRACT:

Background

Receptor tyrosine kinase-like orphan receptor 2 (ROR2) is a Wnt5a receptor aberrantly expressed in cancer that was shown to either suppress or promote carcinogenesis in different tumor types. Our goal was to study the role of ROR2 in melanoma.

Methods

Gain and loss-of-function strategies were applied to study the biological function of ROR2 in melanoma. Proliferation assays, flow cytometry, and western blotting were used to evaluate cell proliferation and changes in expression levels of cell-cycle and proliferation markers. The role of ROR2 in tumor growth was assessed in xenotransplantation experiments followed by immunohistochemistry analysis of the tumors. The role of ROR2 in melanoma patients was assessed by analysis of clinical data from the Leeds Melanoma Co

SUBMITTER: Castro MV 

PROVIDER: S-EPMC8590781 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature