Catechol 1,2-Dioxygenase From Paracoccus sp. MKU1-A Greener and Cleaner Bio-Machinery for cis, cis-Muconic Acid Production by Recombinant E. coli.

ABSTRACT: Cis, cis-muconic acid (ccMA) is known for its industrial importance as a precursor for the synthesis of several biopolymers. Catechol 1,2-dioxygenase (C12O) is involved in aromatic compounds catabolism and ccMA synthesis in a greener and cleaner way. This is the first study on C12O gene from a metabolically versatile Paracoccus sp. MKU1, which was cloned and expressed in E. coli to produce ccMA from catechol. From the E. coli transformant, recombinant C12O enzyme was purified and found to be a homotrimer with a subunit size of 38.6 kDa. The apparent K _m and V _max for C12O was 12.89 µM and 310.1 U.mg^-1, respectively, evidencing high affinity to catechol than previously reported C12Os. The predicted 3D-structur

SUBMITTER: Aravind MK 

PROVIDER: S-EPMC8591083 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature