Single-cell RNA sequencing to characterize the response of pancreatic cancer to anti-PD-1 immunotherapy.

ABSTRACT: Pancreatic cancer (PaC) is resistant to immune checkpoint therapy, but the underlying mechanisms are largely unknown. In this study, we have established four orthotopic PaC murine models with different PaC cell lines by intra-pancreatic inoculation. Therapeutic examinations demonstrate that only tumors induced with Panc02-H7 cells respond to αPD-1 antibody treatment, leading to significantly reduced tumor growth and increased survival in the recipient mice. Transcriptomic profiling at a single-cell resolution characterizes the molecular activity of different cells within tumors. Comparative analysis and validated experiments demonstrate that αPD-1-sensitive and -resistant tumors differently shape the immune landscape in the tumor microenvironment (TME) and markedly altering effector CD8

SUBMITTER: Zhou J 

PROVIDER: S-EPMC8591363 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature