Epistasis, aneuploidy, and functional mutations underlie evolution of resistance to induced microtubule depolymerization.

ABSTRACT: Cells with blocked microtubule polymerization are delayed in mitosis, but eventually manage to proliferate despite substantial chromosome missegregation. While several studies have analyzed the first cell division after microtubule depolymerization, we have asked how cells cope long-term with microtubule impairment. We allowed 24 clonal populations of yeast cells with beta-tubulin mutations preventing proper microtubule polymerization, to evolve for ˜150 generations. At the end of the laboratory evolution experiment, cells had regained the ability to form microtubules and were less sensitive to microtubule-depolymerizing drugs. Whole-genome sequencing identified recurrently mutated genes, in particular for tubulins and kinesins, as well as pervasive duplication of chromosome VIII. Recreati

SUBMITTER: Pavani M 

PROVIDER: S-EPMC8591535 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature