STOML2 interacts with PHB through activating MAPK signaling pathway to promote colorectal Cancer proliferation.

ABSTRACT:

Background

Highly expressed STOML2 has been reported in a variety of cancers, yet few have detailed its function and regulatory mechanism. This research aims to reveal regulatory mechanism of STOML2 and to provide evidence for clinical therapeutics, via exploration of its role in colorectal cancer, and identification of its interacting protein.

Methods

Expression level of STOML2 in normal colon and CRC tissue from biobank in Nanfang Hospital was detected by pathologic methods. The malignant proliferation of CRC induced by STOML2 was validated via gain-of-function and loss-of-function experiments, with novel techniques applied, such as organoid culture, orthotopic model and endoscopy monitoring. Yeast two-hybrid assay screened interacting proteins of STOML2, followed by bioin

SUBMITTER: Ma W 

PROVIDER: S-EPMC8591804 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature