Lamin C is required to establish genome organization after mitosis.

ABSTRACT:

Background

The dynamic 3D organization of the genome is central to gene regulation and development. The nuclear lamina influences genome organization through the tethering of lamina-associated domains (LADs) to the nuclear periphery. Evidence suggests that lamins A and C are the predominant lamins involved in the peripheral association of LADs, potentially serving different roles.

Results

Here, we examine chromosome architecture in mouse cells in which lamin A or lamin C are downregulated. We find that lamin C, and not lamin A, is required for the 3D organization of LADs and overall chromosome organization. Striking differences in localization are present as cells exit mitosis and persist through early G1 and are linked to differential phosphorylation. Whereas lamin A associ

SUBMITTER: Wong X 

PROVIDER: S-EPMC8591896 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature