Evolution of ischemia and neovascularization in a murine model of full thickness human wound healing.

ABSTRACT: Translation of wound healing research is limited by the lack of an appropriate animal model, due to the anatomic and wound healing differences in animals and humans. Here, we characterize healing of grafted, full-thickness human skin in an in vivo model of wound healing. Full-thickness human skin, obtained from reconstructive operations, was grafted onto the dorsal flank of NOD.Cg-Kit^W41J Tyr + Prkdc^scid Il2rg^tm1Wjl /ThomJ mice. The xenografts were harvested 1 to 12 weeks after grafting, and histologic analyses were completed for viability, neovascularization, and hypoxia. Visual inspection of the xenograft shows drying and sloughing of the epidermis starting at week four. By week 12, the xenograft appears healed but has lost 63.05 ± 0.24% of the initial gr

SUBMITTER: Karim AS 

PROVIDER: S-EPMC8592059 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature