Multiplex Bead Assay for the Detection of Human IgG Antibody Responses to African Trypanosomes.

ABSTRACT: The recent introduction of large-scale, population-based serologic surveys in several nations where human African trypanosomiasis (HAT) remains endemic could provide an opportunity to better map the remaining disease foci and to identify asymptomatic, seropositive individuals who are infected with the more chronic form of the parasite, Trypanosoma brucei gambiense (gHAT). We have incorporated a soluble form of variant surface glycoprotein 117 and a recombinant invariant surface glycoprotein 65.1 into a multiplex bead assay (MBA) method that is commonly used for the detection of IgG antibody responses to other neglected tropical diseases. A positive result was defined as reactivity to both antigens. MBA sensitivity and specificity for gHAT infection were 92% and 96%, respectively. Assay spe

SUBMITTER: Priest JW 

PROVIDER: S-EPMC8592197 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature