Contact lenses contamination by Acanthamoeba spp. in Upper Egypt.

ABSTRACT:

Background

Acanthamoeba spp. are one of the free-living amoeba that spread worldwide causing keratitis. Owing to the increase in the use of lenses, whether for medical or cosmetic purposes, the incidence of disease increases every year. Contamination of the lenses with the Acanthamoeba trophozoites or cysts may lead to eye infection and cause sight-threatening keratitis in human. We isolated Acanthamoeba spp. from new lenses, used lenses, and contact lens disinfecting solutions and identified them based on morphological characteristics and molecular test.

Methods

New and used lenses and contact lens disinfecting solutions were cultured on monogenic media. Light and scanning electron microscope was used to identify Acanthamoeba spp. morphological features. Genotype identifica

SUBMITTER: Hassan FAM 

PROVIDER: S-EPMC8592476 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature