Functional interaction of H₂-receptors and 5HT₄-receptors in atrial tissues isolated from double transgenic mice and from human patients.

ABSTRACT: In the past, we generated transgenic mice that overexpress the human histamine 2 (H₂)-receptor (H₂-TG) or that overexpress the human serotonin 4 (5-HT₄)-receptor (5-HT₄-TG) in the heart. Here, we crossbred these lines of mice to generate double transgenic mice that overexpress both receptors (DT). This was done to study a conceivable interaction between these receptors in the mouse heart as a model for the human heart. When in left atria, initially, force of contraction was elevated maximally with 1 µM serotonin, and subsequently, histamine was cumulatively applied; a biphasic effect of histamine was noted: the force of contraction initially decreased, maximally at 10 nM histamine, and thereafter, the force of contraction increased again at 1 µM

SUBMITTER: Neumann J 

PROVIDER: S-EPMC8592968 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature