A low-cost open-source 5-choice operant box system optimized for electrophysiology and optophysiology in mice.

ABSTRACT: Operant boxes enable the application of complex behavioural paradigms to support circuit neuroscience and drug discovery research. However, commercial operant box systems are expensive and often not optimised for combining behaviour with neurophysiology. Here we introduce a fully open-source Python-based operant-box system in a 5-choice design (pyOS-5) that enables assessment of multiple cognitive and affective functions. It is optimized for fast turn-over between animals, and for testing of tethered mice for simultaneous physiological recordings or optogenetic manipulation. For reward delivery, we developed peristaltic and syringe pumps based on a stepper motor and 3D-printed parts. Tasks are specified using a Python-based syntax implemented on custom-designed printed circuit boards that

SUBMITTER: Kapanaiah SKT 

PROVIDER: S-EPMC8593009 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature