Ultrafast identification of Pinelliae Rhizoma using colorimetric direct-VPCR.

ABSTRACT: With the increasing growth of the herbal market, a rapid and easy-to-use system is highly desirable in the high-throughput identification of massive herbal medicine samples. Here, an ultrafast and colorimetric detection system was devised based on simplifying template preparation and a newly developed amplification technique, named colorimetric direct-VPCR. The system was successfully applied to the identification of Pinelliae Rhizoma. Compared to the traditional method, the whole test can be finished within 30 min from the sample treatment to the testing results. The method was evaluated by correctly identifying 72 samples obtained from 9 different habitats, demonstrating its high reliability. In summary, we present an ultrafast (less than 30 min) and colorimetric detection platform (unde

SUBMITTER: Chen R 

PROVIDER: S-EPMC8593080 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature