SARS-CoV-2 detection using reverse transcription strand invasion based amplification and a portable compact size instrument.

ABSTRACT: Rapid nucleic-acid based tests that can be performed by non-professionals outside laboratory settings could help the containment of the pandemic SARS-CoV-2 virus and may potentially prevent further widespread lockdowns. Here, we present a novel compact portable detection instrument (the Egoo Health System) for extraction-free detection of SARS-CoV-2 using isothermal reverse transcription strand invasion based amplification (RT-SIBA). The SARS-CoV-2 RT-SIBA assay can be performed directly on crude oropharyngeal swabs without nucleic acid extraction with a reaction time of 30 min. The Egoo Health system uses a capsule system, which is automatically sealed tight in the Egoo instrument after applying the sample, resulting in a closed system optimal for molecular isothermal amplification. The p

SUBMITTER: Rosenstierne MW 

PROVIDER: S-EPMC8593107 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature