Enumeration of viable CD34+ cells in cord blood using a novel stem cell enumeration kit.

ABSTRACT:

Objective

To assess the detection performance of the hematopoietic stem cell enumeration kit developed by BD Biosciences.

Methods

Cord blood samples were prepared using a hematopoietic stem cell enumeration kit developed by BD Biosciences and Stem-Kit reagents from Beckman Coulter. CD34+ cells were enumerated using a BD FACSCanto instrument and FACSDiva software.

Results

A total of 519 samples were analyzed in this study. The hematopoietic stem cell enumeration kit developed by BD Biosciences yielded absolute counts of CD34-positive cells that were on average 8.7% lower than Beckman Coulter Stem-Kit reagents (range: -5.7% to-14.7%). The BD Biosciences kit yielded relative counts that were on average 9.9% higher compared with Beckman Coulter Stem-Kit reagents (range: -

SUBMITTER: Huang S 

PROVIDER: S-EPMC8593314 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature