A Bacterial Cell-Based Assay To Study SARS-CoV-2 Protein-Protein Interactions.

ABSTRACT: Methods for detecting and dissecting the interactions of virally encoded proteins are essential for probing basic viral biology and providing a foundation for therapeutic advances. The dearth of targeted therapeutics for the treatment of coronavirus disease 2019 (COVID-19), an ongoing global health crisis, underscores the importance of gaining a deeper understanding of the interactions of proteins encoded by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, we describe the use of a convenient bacterial cell-based two-hybrid (B2H) system to analyze the SARS-CoV-2 proteome. We identified 16 distinct intraviral protein-protein interactions (PPIs), involving 16 proteins. We found that many of the identified proteins interact with more than one partner. Further, our system fac

SUBMITTER: Springstein BL 

PROVIDER: S-EPMC8593686 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature